A tetrapeptide has –COOH group on alanine. This produces glycine (Gly), Valine (Val), Phenyl Alanine (Phe) and Alanine (Ala), on complete hydrolysis. For this tetrapeptide, the number of possible sequences (primary structures) with –NH2 group attached to a chiral center is
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Tetrapeptide contains 3 peptide linkage and gives 4 amino acid on hydrolysis

Since tetrapeptide contains free COOH at alanine so that alanine is last unit of tetrapeptide. Glycene does not contain chiral carbon so that Glycene cannot be at first position in tetrapeptide. Hence possible structure of this tetrapeptide will be 4
PVGA VGPA
PGVA VPGA