Centrifugation is used to separate sub-cellular components based on size and density. The greater the density, the faster it move. Differential centrifugation applies different speed in order to separate different sub-cellular component from the homogenate. When a homogenized liver tissue is centrifuged at 500 xg, the heaviest sub-cellular components will settle first, that is nuclei. When the supernatant is again centrifuged at 10000 xg, the second heavier sub-cellular component will settle, that is mitochondria. When the supernatant is centrifuged at very high speed of 25000 xg, the lightest sub-cellular component will settle, that is lysosomes.